Bacillus anthracis hemliga liv

Bacillus anthracis is the causative agent of anthrax, a serious and globally distributed zoonosis affecting a wide range of wild and domestic animals, invariably also humans. However, although known to humans since biblical times, much remains to be elucidated concerning the ecology and transmission of this bacterium. Of particular interest is the Bacillus anthracis spore, the uptake of which is the predominant way to contract anthrax and which is legendary for its resilience in the environment and thus crucial for persistence and spread of the disease. Hence, the aim of this study is to review the natural transmission of Bacillus anthracis and investigate potential means by which soil persisting Bacillus anthracis spores reach concentrations sufficient to infect susceptible hosts. When reviewing the literature, three different theories can be distinguished. Firstly, “the incubator area” hypothesis suggests that favourable soil factors, possibly in association with amoebas, may constitute an environment supporting repeated spore-bacterium-spore cycling, thus increasing the local amount of spores. Secondly, water runoff from heavy rains or flooding has been proposed to collect spores and dispose them in closely restricted sites, thus creating “concentrator areas” with locally high amounts of spores. Lastly, the outermost layer of the spore, the exosporium, is proposed to tie spores to the environment where they were first shed and hence maintain infectious spore concentrations at a particular site. Considering that these theories all have their agreements and disagreements with the ecology and epidemiology of anthrax, it is reasonable to assume that all three exert an impact on spore concentrations, possibly at different degrees at various sites and regions. Howsoever, the ecology of Bacillus anthracis requires further research to fully understand the mechanism responsible for transmission and spread of anthrax. Only then can efficient methods for eradication of the disease from contaminated fields, and hence reducing the risk of future epidemics, be developed.Bacillus anthracis är en zoonotisk bakterie som orsakar den allvarliga och globalt förekommande sjukdomen anthrax, eller mjältbrand. Trots att både agens och sjukdom är välbekanta sedan antikens dagar och har varit i fokus för gedigen forskning i över 150 år, kvarstår häpnadsväckande många frågetecken. Av särskilt intresse är Bacillus anthracis alternerande livscykel mellan vegetativ, replikerande cell och metaboliskt vilande, men fortfarande infektiös, spor. Då sporerna har en närmast legendarisk motståndskraft mot yttre omgivningsfaktorer kan de kvarstå i miljön under mycket lång tid, och således upprätthålla ett infektiöst fokus av Bacillus anthracis i jordmånen, från vilket nya mottagliga individer kan infekteras. Då anthrax är en sjukdom som huvudsakligen smittar via sporer och har en hög infektionsdos är syftet med denna litteraturöversikt följaktligen att utröna hur infektiösa nivåer av sporer bildas och bibehållas i omgivningen, samt undersöka möjliga spridningsvägar för nämnda sporer. I litteraturen framträder tre huvudsakliga teorier angående hur sporer koncentreras: För det första, i den så kallade ”incubator area”-hypotesen föreslås Bacillus anthracis sporer uppnå höga antal genom att självständigt, eller möjligtvis via amöbor, germinera, replikera och re-sporulera i miljön, dvs. utanför ett värddjur, under förutsättning att yttre faktorer är gynnsamma. Ett ytterligare alternativ förs fram i ”concentrator area”-teorin där Bacillus anthracis sporer tros följa med avrinningsvatten efter skyfall eller översvämningar för att ackumuleras i låglänta områden där höga lokala sporkoncentrationer således upprättas. Slutligen framförs möjligheten att Bacillus anthracis sporernas yttersta hölje, exosporium, binder sporerna till jordpartiklar och följaktligen kvarhålls de på det ställe där de först hamnar efter bildning. Under beaktande att alla tre teorier uppvisar både enigheter och motstridigheter med Bacillus anthracis epidemiologi och ekologi, är det rimligt att anta att de alla, var och en på sitt sätt, bidrar till att etablera och bibehålla infektiösa spormängder, troligtvis av varierande grad i olika miljöer och regioner. Dock kvarstår mycket forskning för att fullt kunna kartlägga Bacillus anthracis komplexa ekologi och därmed möjliggöra utvecklingen av effektiva metoder för sanering av sporkontaminerade områden och således förebygga framtida anthrax epidemier

Soil moisture remote-sensing applications for identification of flood-prone areas along transport infrastructure

AbstractThe expected increase in precipitation and temperature in Scandinavia, and especially short-time heavy precipitation, will increase the frequency of flooding. Urban areas are the most vulnerable, and specifically, the road infrastructure. The accumulation of large volumes of water and sediments on road-stream intersections gets severe consequences for the road drainage structures. This study integrates the spatial and temporal soil moisture properties into the research about flood prediction methods by a case study of two areas in Sweden, Västra Götaland and Värmland, which was affected by severe flooding in August 2014. Soil moisture data are derived from remote-sensing techniques, with a focus on the soil moisture-specific satellites ASCAT and SMOS. Furthermore, several physical catchments descriptors (PCDs) are analyzed and the result shows that larger slopes and drainage density, in general, mean a higher risk of flooding. The precipitation is the same; however, it can be concluded that more precipitation in most cases gives higher soil moisture values. The lack, or the dimensioning, of road drainage structures seems to have a large impact on the flood risk as more sediment and water can be accumulated at the road-stream intersection. The results show that the method implementing soil moisture satellite data is promising for improving the reliability of flooding

Feline morbillivirus infection associated with fatal encephalitis in a Bengal cat.

Feline morbillivirus (FeMV) is a recently discovered morbillivirus of the family Paramyxoviridae, which include several highly contagious viruses with zoonotic potential. In this case report we describe the detection of FeMV in archived brain tissue of a 2-month-old Bengal cat with nonsuppurative encephalitis from the year 2011 in Switzerland by high-throughput sequencing (HTS). Our metagenomics approach was able to obtain a full-length sequence covering the entire FeMV genome. Phylogenetic analysis showed that our FeMV strain clustered within FeMV genotype 1. We were able to detect FeMV RNA by in situ hybridization (ISH) in brain sections with inflammatory lesions and demonstrated its potential neurotropism and association with encephalitis. Our results provide further insight into this recently discovered morbillivirus and encourage further investigations into the pathogenesis and epidemiology of associated diseases in cats and potentially other species

Exploring chitosan-shelled nanobubbles to improve HER2 + immunotherapy via dendritic cell targeting

Immunotherapy is a valuable approach to cancer treatment as it is able to activate the immune system. However, the curative methods currently in clinical practice, including immune checkpoint inhibitors, present some limitations. Dendritic cell vaccination has been investigated as an immunotherapeutic strategy, and nanotechnology-based delivery systems have emerged as powerful tools for improving immunotherapy and vaccine development. A number of nanodelivery systems have therefore been proposed to promote cancer immunotherapy. This work aims to design a novel immunotherapy nanoplatform for the treatment of HER2 + breast cancer, and specially tailored chitosan-shelled nanobubbles (NBs) have been developed for the delivery of a DNA vaccine. The NBs have been functionalized with anti-CD1a antibodies to target dendritic cells (DCs). The NB formulations possess dimensions of approximately 300 nm and positive surface charge, and also show good physical stability up to 6 months under storage at 4 °C. In vitro characterization has confirmed that these NBs are capable of loading DNA with good encapsulation efficiency (82%). The antiCD1a-functionalized NBs are designed to target DCs, and demonstrated the ability to induce DC activation in both human and mouse cell models, and also elicited a specific immune response that was capable of slowing tumor growth in mice in vivo. These findings are the proof of concept that loading a tumor vaccine into DC-targeted chitosan nanobubbles may become an attractive nanotechnology approach for the future immunotherapeutic treatment of cancer. GRAPHICAL ABSTRACT: [Image: see text

Nanotechnology Addressing Cutaneous Melanoma: The Italian Landscape

Cutaneous melanoma is one of the most aggressive solid tumors, with a low survival for the metastatic stage. Currently, clinical melanoma treatments include surgery, chemotherapy, targeted therapy, immunotherapy and radiotherapy. Of note, innovative therapeutic regimens concern the administration of multitarget drugs in tandem, in order to improve therapeutic efficacy. However, also, if this drug combination is clinically relevant, the patient’s response is not yet optimal. In this scenario, nanotechnology-based delivery systems can play a crucial role in the clinical treatment of advanced melanoma. In fact, their nano-features enable targeted drug delivery at a cellular level by overcoming biological barriers. Various nanomedicines have been proposed for the treatment of cutaneous melanoma, and a relevant number of them are undergoing clinical trials. In Italy, researchers are focusing on the pharmaceutical development of nanoformulations for malignant melanoma therapy. The present review reports an overview of the main melanoma-addressed nanomedicines currently under study in Italy, alongside the state of the art of melanoma therapy. Moreover, the latest Italian advances concerning the pre-clinical evaluation of nanomedicines for melanoma are described

Polycomb Controls Gliogenesis by Regulating the Transient Expression of the Gcm/Glide Fate Determinant

The Gcm/Glide transcription factor is transiently expressed and required in the Drosophila nervous system. Threshold Gcm/Glide levels control the glial versus neuronal fate choice, and its perdurance triggers excessive gliogenesis, showing that its tight and dynamic regulation ensures the proper balance between neurons and glia. Here, we present a genetic screen for potential gcm/glide interactors and identify genes encoding chromatin factors of the Trithorax and of the Polycomb groups. These proteins maintain the heritable epigenetic state, among others, of HOX genes throughout development, but their regulatory role on transiently expressed genes remains elusive. Here we show that Polycomb negatively affects Gcm/Glide autoregulation, a positive feedback loop that allows timely accumulation of Gcm/Glide threshold levels. Such temporal fine-tuning of gene expression tightly controls gliogenesis. This work performed at the levels of individual cells reveals an undescribed mode of Polycomb action in the modulation of transiently expressed fate determinants and hence in the acquisition of specific cell identity in the nervous system. © 2012 Popkova et al.Fondation pour la Recherche Médicale and by Centre Européen de Recherche en Biologie et en Médecine; Association pour la Recherche sur le Cancer; Institut National de la Santé et de la Recherche Médicale; Centre National de la Recherche Scientifique; Université de Strasbourg; Hôpital de Strasbourg; Institut National du Cancer; the Agence Nationale de la Recherche; Alma Mater Studiorum; Università di Bologna; European Research Council (ERC-2008-AdG No 232947); Institut National de la Santé et de la Recherche Médicale; Centre National de la Recherche Scientifique; European Network of Excellence EpiGeneSys; Fundacion Mutua Madrileña (FMM-2006) and Ministerio de Ciencia y Tecnología (BFU-2008-5404)Peer Reviewe

Occupational Therapists and COVID-19 Pandemic: An Observational Survey in Europe

Background: The COVID-19 pandemic has resulted in a health care emergency in Europe since the first wave in 2020. Several challenges have arisen for occupational therapists, as well as all the health care professionals. The aim of this study was to determine what occupational therapists have changed to adapt their therapeutic processes for this catastrophic situation. Method: An online survey was developed and sent in conjunction with the Council of Occupational Therapy for European Countries (COTEC) to European national associations of occupational therapists. Results: The study was based on a sample of 65 occupational therapists who worked with people with COVID-19. More than half of the occupational therapists (54.8%) had changed departments. The main needs patients expressed (n = 136) during hospitalization were to have social contacts (30.9%), and the main clinical complaints (n = 144) were motor impairment and fatigue (35.4%) and depression (25.7%). The most frequently reported goal (n = 141) was recovery of physical performance and fatigue management (32.6%). Among the emotions mentioned by occupational therapists, negative emotions (76%) were the most common. Conclusion: European occupational therapists demonstrated flexibility and resilience to deal with clinical and organizational challenges during the COVID-19 emergency

Multitarget drug design strategy in Alzheimer’s disease: focus on cholinergic transmission and amyloid-β aggregation

Background: Alzheimer pathogenesis has been associated with a network of processes working simultaneously and synergistically. Over time, much interest has been focused on cholinergic transmission and its mutual interconnections with other active players of the disease. Besides the cholinesterase mainstay, the multifaceted interplay between nicotinic receptors and amyloid is actually considered to have a central role in neuroprotection. Thus, the multitarget drug-design strategy has emerged as a chance to face the disease network. Results: By exploiting the multitarget approach, the present study provides new molecules able to target the cholinergic pathway, by joining direct nicotinic receptor stimulation to acetylcholinesterase inhibition, and to inhibit Aβ aggregation. Conclusions: These new compounds emerged as a suitable starting point for a further optimization process